Description
CST's PathScan® Di-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit #7124 detects endogenous levels of histone H3 when di-methylated at Lys4. As shown in Figure 1 using the Di-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit #7124, a high level of di-methylation at Lys4 on histone H3 is detected in COS cells when treated with TSA. The level of total histone H3 (modified and unmodified) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when NIH/3T3 and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species